##Sketch 1 2
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Fn('Times-Roman')
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G_()
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Fn('Times-Roman')
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Fn('Times-Roman')
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Fn('Times-Roman')
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Fn('Times-Roman')
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Fn('Times-Roman')
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Fn('Times-Roman')
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Fn('Times-Roman')
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Fn('Times-Roman')
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fp((0,0,0))
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Fn('Times-Roman')
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G_()
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Fn('Times-Roman')
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Fn('Times-Roman')
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lw(1)
Fn('Times-Roman')
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fp((0,0,0))
le()
lw(1)
Fn('Times-Roman')
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G()
lw(1)
b()
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lw(1)
b()
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G_()
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le()
lw(1)
Fn('Times-Roman')
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G()
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Fn('Times-Roman')
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fp((0,0,0))
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lw(1)
Fn('Times-Roman')
txt('O',(0.757118,0,0,0.757118,398.868,401.798))
fp((0,0,0))
le()
lw(1)
Fn('Times-Roman')
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fp((0,0,0))
le()
lw(1)
Fn('Times-Roman')
txt('OH',(0.757118,0,0,0.757118,375.878,386.471))
fp((0,0,0))
le()
lw(1)
Fn('Times-Roman')
txt('P',(0.757118,0,0,0.757118,377.013,401.798))
G()
lw(1)
b()
bs(378.167,416.274,0)
bs(378.172,410.239,0)
bs(378.171,410.775,0)
lw(1)
b()
bs(380.201,416.274,0)
bs(380.206,410.239,0)
bs(380.206,410.775,0)
G_()
G_()
lw(1)
b()
bs(117.513,309.53,0)
bs(126.768,309.53,0)
bs(125.945,309.53,0)
lw(1)
b()
bs(318.071,309.227,0)
bs(327.326,309.227,0)
bs(326.503,309.227,0)
lw(1)
b()
bs(338.08,309.227,0)
bs(347.335,309.227,0)
bs(346.512,309.227,0)
lw(1)
b()
bs(110.72,304.399,0)
bs(110.725,298.364,0)
bs(110.724,298.9,0)
lw(1)
b()
bs(332.24,304.096,0)
bs(332.245,298.061,0)
bs(332.244,298.597,0)
fp((0,0,0))
le()
lw(1)
Fn('Times-Roman')
txt('O',(0.757118,0,0,0.757118,130.38,306.496))
fp((0,0,0))
le()
lw(1)
Fn('Times-Roman')
txt('O',(0.757118,0,0,0.757118,351.9,306.193))
fp((0,0,0))
le()
lw(1)
Fn('Times-Roman')
txt('HO',(0.757118,0,0,0.757118,302.354,306.193))
fp((0,0,0))
le()
lw(1)
Fn('Times-Roman')
txt('O',(0.757118,0,0,0.757118,107.39,321.956))
fp((0,0,0))
le()
lw(1)
Fn('Times-Roman')
txt('O',(0.757118,0,0,0.757118,328.91,321.653))
fp((0,0,0))
le()
lw(1)
Fn('Times-Roman')
txt('OH',(0.757118,0,0,0.757118,107.39,291.169))
fp((0,0,0))
le()
lw(1)
Fn('Times-Roman')
txt('OH',(0.757118,0,0,0.757118,328.91,290.866))
fp((0,0,0))
le()
lw(1)
Fn('Times-Roman')
txt('P',(0.757118,0,0,0.757118,108.525,306.496))
fp((0,0,0))
le()
lw(1)
Fn('Times-Roman')
txt('P',(0.757118,0,0,0.757118,330.045,306.193))
G()
lw(1)
b()
bs(109.679,320.972,0)
bs(109.684,314.937,0)
bs(109.683,315.473,0)
lw(1)
b()
bs(111.713,320.972,0)
bs(111.718,314.937,0)
bs(111.718,315.473,0)
G_()
G()
lw(1)
b()
bs(331.199,320.669,0)
bs(331.204,314.634,0)
bs(331.203,315.17,0)
lw(1)
b()
bs(333.233,320.669,0)
bs(333.238,314.634,0)
bs(333.238,315.17,0)
G_()
fp((0,0,0))
le()
lw(1)
Fn('Times-Roman')
txt('OH',(0.757118,0,0,0.757118,446.526,652.513))
fp((0,0,0))
le()
lw(1)
Fn('Times-Roman')
txt('OH',(0.757118,0,0,0.757118,439.865,552.119))
fp((0,0,0))
le()
lw(1)
Fn('Times-Roman')
txt('OH',(0.757118,0,0,0.757118,439.865,452.024))
fp((0,0,0))
le()
lw(1)
Fn('Times-Roman')
txt('OH',(0.757118,0,0,0.757118,439.865,501.893))
fp((0,0,0))
le()
lw(1)
Fn('Times-Roman')
txt('OH',(0.757118,0,0,0.757118,439.865,401.798))
fp((0,0,0))
le()
lw(1)
Fn('Times-Roman')
txt('O',(0.757118,0,0,0.757118,171.377,306.496))
fp((0,0,0))
le()
lw(1)
Fn('Times-Roman')
txt('O',(0.757118,0,0,0.757118,392.897,306.193))
fp((0,0,0))
le()
lw(1)
Fn('Times-Roman')
txt('a1',(150.581,606.461))
fp((0,0,0))
le()
lw(1)
Fn('Times-Roman')
txt('z1',(474.389,552.374))
fp((0,0,0))
le()
lw(1)
Fn('Times-Roman')
txt('x1',(475.102,454.06))
fp((0,0,0))
le()
lw(1)
Fn('Times-Roman')
txt('w1',(475.102,403.834))
fp((0,0,0))
le()
lw(1)
Fn('Times-Roman')
txt('y1',(475.102,503.93))
fp((0,0,0))
le()
lw(1)
Fn('Times-Roman')
txt('b1',(164.191,602.186))
fp((0,0,0))
le()
lw(1)
Fn('Times-Roman')
txt('c1',(197.53,598.1))
fp((0,0,0))
le()
lw(1)
Fn('Times-Roman')
txt('d1',(212.315,594.17))
fp((0,0,0))
le()
lw(1)
Fn('Times-Roman')
txt('x1',(390.884,695.823))
fp((0,0,0))
le()
lw(1)
Fn('Times-Roman')
txt('y1',(422.192,695.02))
fp((0,0,0))
le()
lw(1)
Fn('Times-Roman')
txt('z1',(436.091,688.684))
fp((0,0,0))
le()
lw(1)
Fn('Times-Roman')
txt('w1',(373.297,700.098))
fp((0,0,0))
le()
lw(1)
Fn('Times-Roman')
txt('Canonical monomer',(84.8986,338.347))
fp((0,0,0))
le()
lw(1)
Fn('Times-Roman')
txt('Normally, the left end of a nucleic acid, after capping, is:',(210.789,338.347))
fp((0,0,0))
le()
lw(1)
Fn('Times-Roman')
txt("Thus, if 5'OH is required, subtract   HPO3   from",(223.24,267.386))
fp((0,0,0))
le()
lw(1)
Fn('Times-Roman')
txt('left end-bearing fragments (like a, b, c, d)',(222.786,253.538))
lw(1)
la2(([(-4.0, 3.0), (2.0, 0.0), (-4.0, -3.0), (-4.0, 3.0)], 1))
b()
bs(211.68,307.342,0)
bs(270.049,307.342,0)
fp((0,0,0))
le()
lw(1)
Fn('Times-Roman')
txt('+ OH',(222.625,311.902))
lw(1)
ld((0.1, 1.0))
r(39.6727,0,0,-45.1448,320.21,331.966)
lw(1)
ld((0.1, 1.0))
r(39.6727,0,0,-14.5923,392.715,278.732)
fp((0,0,0))
le()
lw(1)
Fn('Times-Roman')
txt('All the fragments below bear one negative charge',(157.786,728.096))
fp((0,0,0))
le()
lw(1)
Fn('Times-Roman')
txt('(not formally represented on the sequence/fragments because it can be floating at any valid place)',(0.667197,0,0,0.667199,124.045,717.52))
lw(1)
la2(([(-4.0, 3.0), (2.0, 0.0), (-4.0, -3.0), (-4.0, 3.0)], 1))
b()
bs(411.545,555.338,0)
bs(402.831,562.096,1)
bs(394.338,555.471,1)
bs(381.456,555.471,0)
lw(1)
la2(([(-4.0, 3.0), (2.0, 0.0), (-4.0, -3.0), (-4.0, 3.0)], 1))
b()
bs(392.211,505.071,0)
bs(383.497,511.828,1)
bs(375.004,505.203,1)
bs(362.123,505.203,0)
lw(1)
la2(([(-4.0, 3.0), (2.0, 0.0), (-4.0, -3.0), (-4.0, 3.0)], 1))
b()
bs(370.461,454.803,0)
bs(361.747,461.561,1)
bs(353.254,454.936,1)
bs(340.372,454.936,0)
lw(1)
la2(([(-4.0, 3.0), (2.0, 0.0), (-4.0, -3.0), (-4.0, 3.0)], 1))
b()
bs(348.227,404.079,0)
bs(339.513,410.837,1)
bs(331.02,404.212,1)
bs(318.139,404.212,0)
G()
lw(1)
b()
bs(179.66,521.41,0)
bs(179.66,486.374,0)
bs(173.107,505.06,0)
bs(163.278,505.06,0)
lw(1)
b()
bs(179.66,571.28,0)
bs(179.66,536.243,0)
bs(173.107,554.929,0)
bs(163.278,554.929,0)
lw(1)
b()
bs(179.66,471.185,0)
bs(179.66,436.148,0)
bs(173.107,454.834,0)
bs(163.278,454.834,0)
lw(1)
b()
bs(179.66,420.603,0)
bs(179.66,385.566,0)
bs(173.107,404.252,0)
bs(163.278,404.252,0)
lw(1)
b()
bs(179.66,521.41,0)
bs(179.66,486.374,0)
bs(173.107,505.06,0)
bs(163.278,505.06,0)
lw(1)
b()
bs(179.66,571.28,0)
bs(179.66,536.243,0)
bs(173.107,554.929,0)
bs(163.278,554.929,0)
lw(1)
b()
bs(179.66,471.185,0)
bs(179.66,436.148,0)
bs(173.107,454.834,0)
bs(163.278,454.834,0)
lw(1)
b()
bs(179.66,420.603,0)
bs(179.66,385.566,0)
bs(173.107,404.252,0)
bs(163.278,404.252,0)
lw(1)
b()
bs(179.886,505.074,0)
bs(189.863,505.074,0)
bs(188.976,505.074,0)
lw(1)
b()
bs(179.886,454.849,0)
bs(189.863,454.849,0)
bs(188.976,454.849,0)
lw(1)
b()
bs(179.886,404.267,0)
bs(189.863,404.267,0)
bs(188.976,404.267,0)
lw(1)
b()
bs(116.476,505.074,0)
bs(125.731,505.074,0)
bs(124.908,505.074,0)
lw(1)
b()
bs(116.476,554.945,0)
bs(125.731,554.945,0)
bs(124.908,554.945,0)
lw(1)
b()
bs(116.476,454.849,0)
bs(125.731,454.849,0)
bs(124.908,454.849,0)
lw(1)
b()
bs(116.476,404.267,0)
bs(125.731,404.267,0)
bs(124.908,404.267,0)
lw(1)
b()
bs(139.412,505.074,0)
bs(148.667,505.074,0)
bs(147.845,505.074,0)
lw(1)
b()
bs(139.412,554.945,0)
bs(148.667,554.945,0)
bs(147.845,554.945,0)
lw(1)
b()
bs(139.412,454.849,0)
bs(148.667,454.849,0)
bs(147.845,454.849,0)
lw(1)
b()
bs(139.412,404.267,0)
bs(148.667,404.267,0)
bs(147.845,404.267,0)
lw(1)
b()
bs(132.619,499.943,0)
bs(132.624,493.908,0)
bs(132.624,494.444,0)
lw(1)
b()
bs(132.619,549.812,0)
bs(132.624,543.777,0)
bs(132.624,544.314,0)
lw(1)
b()
bs(132.619,449.717,0)
bs(132.624,443.682,0)
bs(132.624,444.219,0)
lw(1)
b()
bs(132.619,399.135,0)
bs(132.624,393.1,0)
bs(132.624,393.637,0)
fp((0,0,0))
le()
lw(1)
Fn('Times-Roman')
txt('HO',(0.757118,0,0,0.757118,100.279,551.91))
fp((0,0,0))
le()
lw(1)
Fn('Times-Roman')
txt('HO',(0.757118,0,0,0.757118,100.279,501.95))
fp((0,0,0))
le()
lw(1)
Fn('Times-Roman')
txt('HO',(0.757118,0,0,0.757118,100.279,451.654))
fp((0,0,0))
le()
lw(1)
Fn('Times-Roman')
txt('HO',(0.757118,0,0,0.757118,100.279,401.105))
fp((0,0,0))
le()
lw(1)
Fn('Times-Roman')
txt('O',(0.757118,0,0,0.757118,152.28,502.04))
fp((0,0,0))
le()
lw(1)
Fn('Times-Roman')
txt('O',(0.757118,0,0,0.757118,152.28,551.91))
fp((0,0,0))
le()
lw(1)
Fn('Times-Roman')
txt('O',(0.757118,0,0,0.757118,152.28,451.814))
fp((0,0,0))
le()
lw(1)
Fn('Times-Roman')
txt('O',(0.757118,0,0,0.757118,152.28,401.233))
fp((0,0,0))
le()
lw(1)
Fn('Times-Roman')
txt('O',(0.757118,0,0,0.757118,129.289,517.5))
fp((0,0,0))
le()
lw(1)
Fn('Times-Roman')
txt('O',(0.757118,0,0,0.757118,129.289,567.37))
fp((0,0,0))
le()
lw(1)
Fn('Times-Roman')
txt('O',(0.757118,0,0,0.757118,129.289,467.275))
fp((0,0,0))
le()
lw(1)
Fn('Times-Roman')
txt('O',(0.757118,0,0,0.757118,129.289,416.693))
fp((0,0,0))
le()
lw(1)
Fn('Times-Roman')
txt('OH',(0.757118,0,0,0.757118,129.289,486.713))
fp((0,0,0))
le()
lw(1)
Fn('Times-Roman')
txt('OH',(0.757118,0,0,0.757118,129.289,536.583))
fp((0,0,0))
le()
lw(1)
Fn('Times-Roman')
txt('OH',(0.757118,0,0,0.757118,129.289,436.488))
fp((0,0,0))
le()
lw(1)
Fn('Times-Roman')
txt('OH',(0.757118,0,0,0.757118,129.289,385.906))
fp((0,0,0))
le()
lw(1)
Fn('Times-Roman')
txt('P',(0.757118,0,0,0.757118,130.425,502.04))
fp((0,0,0))
le()
lw(1)
Fn('Times-Roman')
txt('P',(0.757118,0,0,0.757118,130.425,551.91))
fp((0,0,0))
le()
lw(1)
Fn('Times-Roman')
txt('P',(0.757118,0,0,0.757118,130.425,451.814))
fp((0,0,0))
le()
lw(1)
Fn('Times-Roman')
txt('P',(0.757118,0,0,0.757118,130.425,401.233))
G()
lw(1)
b()
bs(131.578,516.516,0)
bs(131.583,510.481,0)
bs(131.583,511.017,0)
lw(1)
b()
bs(133.613,516.516,0)
bs(133.618,510.481,0)
bs(133.618,511.017,0)
G_()
G()
lw(1)
b()
bs(131.578,566.385,0)
bs(131.583,560.35,0)
bs(131.583,560.887,0)
lw(1)
b()
bs(133.613,566.385,0)
bs(133.618,560.35,0)
bs(133.618,560.887,0)
G_()
G()
lw(1)
b()
bs(131.578,466.29,0)
bs(131.583,460.255,0)
bs(131.583,460.791,0)
lw(1)
b()
bs(133.613,466.29,0)
bs(133.618,460.255,0)
bs(133.618,460.791,0)
G_()
G()
lw(1)
b()
bs(131.578,415.708,0)
bs(131.583,409.673,0)
bs(131.583,410.21,0)
lw(1)
b()
bs(133.613,415.708,0)
bs(133.618,409.673,0)
bs(133.618,410.21,0)
G_()
fp((0,0,0))
le()
lw(1)
Fn('Times-Roman')
txt('B1',(0.757118,0,0,0.757118,176.758,523.24))
fp((0,0,0))
le()
lw(1)
Fn('Times-Roman')
txt('B1',(0.757118,0,0,0.757118,176.758,573.109))
fp((0,0,0))
le()
lw(1)
Fn('Times-Roman')
txt('B1',(0.757118,0,0,0.757118,176.758,473.014))
fp((0,0,0))
le()
lw(1)
Fn('Times-Roman')
txt('B1',(0.757118,0,0,0.757118,176.758,422.432))
fp((0,0,0))
le()
lw(1)
Fn('Times-Roman')
txt("5'",(0.881001,0,0,0.881001,89.8405,550.861))
fp((0,0,0))
le()
lw(1)
Fn('Times-Roman')
txt("5'",(0.881001,0,0,0.881001,89.8405,500.901))
fp((0,0,0))
le()
lw(1)
Fn('Times-Roman')
txt("5'",(0.881001,0,0,0.881001,89.8405,450.605))
fp((0,0,0))
le()
lw(1)
Fn('Times-Roman')
txt("5'",(0.881001,0,0,0.881001,89.8405,400.056))
fp((0,0,0))
le()
lw(1)
Fn('Times-Roman')
txt('O',(0.757118,0,0,0.757118,194.36,502.04))
lw(1)
b()
bs(204.248,454.849,0)
bs(213.503,454.849,0)
bs(212.681,454.849,0)
lw(1)
b()
bs(220.392,449.717,0)
bs(220.397,443.682,0)
bs(220.396,444.219,0)
fp((0,0,0))
le()
lw(1)
Fn('Times-Roman')
txt('O',(0.757118,0,0,0.757118,194.36,451.814))
fp((0,0,0))
le()
lw(1)
Fn('Times-Roman')
txt('O',(0.757118,0,0,0.757118,217.062,467.275))
fp((0,0,0))
le()
lw(1)
Fn('Times-Roman')
txt('OH',(0.757118,0,0,0.757118,217.062,436.488))
fp((0,0,0))
le()
lw(1)
Fn('Times-Roman')
txt('P',(0.757118,0,0,0.757118,218.197,451.814))
G()
lw(1)
b()
bs(219.351,466.29,0)
bs(219.356,460.255,0)
bs(219.355,460.791,0)
lw(1)
b()
bs(221.385,466.29,0)
bs(221.39,460.255,0)
bs(221.39,460.791,0)
G_()
G()
lw(1)
b()
bs(204.248,404.267,0)
bs(213.503,404.267,0)
bs(212.681,404.267,0)
lw(1)
b()
bs(227.185,404.267,0)
bs(236.44,404.267,0)
bs(235.617,404.267,0)
lw(1)
b()
bs(220.392,399.135,0)
bs(220.397,393.1,0)
bs(220.396,393.637,0)
fp((0,0,0))
le()
lw(1)
Fn('Times-Roman')
txt('O',(0.757118,0,0,0.757118,194.36,401.233))
fp((0,0,0))
le()
lw(1)
Fn('Times-Roman')
txt('O',(0.757118,0,0,0.757118,240.052,401.233))
fp((0,0,0))
le()
lw(1)
Fn('Times-Roman')
txt('O',(0.757118,0,0,0.757118,217.062,416.693))
fp((0,0,0))
le()
lw(1)
Fn('Times-Roman')
txt('OH',(0.757118,0,0,0.757118,217.062,385.906))
fp((0,0,0))
le()
lw(1)
Fn('Times-Roman')
txt('P',(0.757118,0,0,0.757118,218.197,401.233))
G()
lw(1)
b()
bs(219.351,415.708,0)
bs(219.356,409.673,0)
bs(219.355,410.21,0)
lw(1)
b()
bs(221.385,415.708,0)
bs(221.39,409.673,0)
bs(221.39,410.21,0)
G_()
G_()
fp((0,0,0))
le()
lw(1)
Fn('Times-Roman')
txt('a1',(69.2065,551.096))
fp((0,0,0))
le()
lw(1)
Fn('Times-Roman')
txt('c1',(69.9195,452.782))
fp((0,0,0))
le()
lw(1)
Fn('Times-Roman')
txt('d1',(69.9195,402.556))
fp((0,0,0))
le()
lw(1)
Fn('Times-Roman')
txt('b1',(69.9195,502.651))
lw(1)
la2(([(-4.0, 3.0), (2.0, 0.0), (-4.0, -3.0), (-4.0, 3.0)], 1))
b()
bs(192.108,555.338,0)
bs(201.483,561.963,1)
bs(210.483,555.338,1)
bs(223.046,555.338,0)
bs(223.046,555.338,0)
lw(1)
la2(([(-4.0, 3.0), (2.0, 0.0), (-4.0, -3.0), (-4.0, 3.0)], 1))
b()
bs(208.059,505.554,0)
bs(217.434,512.179,1)
bs(226.434,505.554,1)
bs(238.996,505.554,0)
bs(238.996,505.554,0)
lw(1)
la2(([(-4.0, 3.0), (2.0, 0.0), (-4.0, -3.0), (-4.0, 3.0)], 1))
b()
bs(231.259,454.803,0)
bs(240.634,461.428,1)
bs(249.634,454.803,1)
bs(262.196,454.803,0)
bs(262.196,454.803,0)
lw(1)
la2(([(-4.0, 3.0), (2.0, 0.0), (-4.0, -3.0), (-4.0, 3.0)], 1))
b()
bs(253.009,404.052,0)
bs(262.384,410.677,1)
bs(271.384,404.052,1)
bs(283.947,404.052,0)
bs(283.947,404.052,0)
G_()
guidelayer('Guide Lines',1,0,0,1,(0,0,1))
grid((0,0,20,20),0,(0,0,1),'Grid')